Mostrando 10 resultados de: 10
Filtros aplicados
Publisher
Electrophoresis(3)
Analytical Biochemistry(1)
Biomolecules(1)
ELECTROPHORESIS(1)
Frontiers in Immunology(1)
Área temáticas
Bioquímica(5)
Farmacología y terapéutica(4)
Química analítica(4)
Enfermedades(3)
Fisiología humana(3)
Origen
scopus(10)
A procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusDestroy to Rebuild: The Connection Between Bone Tissue Remodeling and Matrix Metalloproteinases
ReviewAbstract: Bone is a dynamic organ that undergoes constant remodeling, an energetically costly process by whichPalabras claves:bone, deficiency, Matrix metalloproteinase, metabolism, remodeling, underactivityAutores:Eugenio Hardy, Fernandez‐Patron C.Fuentes:scopusGlucocorticoids Bind to SARS-CoV-2 S1 at Multiple Sites Causing Cooperative Inhibition of SARS-CoV-2 S1 Interaction With ACE2
ArticleAbstract: Dexamethasone may reduce mortality in COVID-19 patients. Whether dexamethasone or endogenous glucocoPalabras claves:ACE2 (angiotensin converting enzyme 2), CORONAVIRUS, covid, glucocorticoids, innate immunity, SARS-COV-2Autores:Elahi S., Eugenio Hardy, Fernandez‐Patron C., Glover J.N.M., Panigrahi R., Sarker H.Fuentes:scopusHigh yield elution of proteins from sodium dodecyl sulfate- polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in-solution biological activity analysis of on-gel renatured proteins
Conference ObjectAbstract: A simple, reliable procedure for practically quantitative (90-98%) and fast (<30 min) elution of proPalabras claves:Passive elution, Protein detection, Protein micropurification, SDS PAGE, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V., Santana-Milián H.Fuentes:scopusMatrix Metalloproteinases in Health and Disease in the Times of COVID-19
OtherAbstract:Palabras claves:Autores:Eugenio Hardy, Fernandez‐Patron C.Fuentes:scopusIdentification of fibrinogen as a natural inhibitor of MMP-2
ArticleAbstract: Non-genetic MMP-2 insufficiency is a relatively unexplored condition which could be induced by pathoPalabras claves:Autores:Botto L.D., Eugenio Hardy, Fernandez‐Patron C., Haimour A., Maksymowych W.P., Sarker H.Fuentes:scopusUnderstanding the mechanism of the zinc-ion stains of biomacromolecules in electrophoresis gels: Generalization of the reverse-staining technique
ArticleAbstract: We have recently shown that a few nanograms of protein separated by electrophoresis in sodium dodecyPalabras claves:detection, Imidazole, inflammation, Lysozyme, Negative stainingAutores:Castellanos‐Serra L., Estevez E., Eugenio Hardy, Fernandez‐Patron C., Frank R., Guerra M., Mehl E.Fuentes:scopusSingle‐step electrotransfer of reverse‐stained proteins from sodium dodecyl sulfate‐polyacrylamide gel onto reversed‐phase minicartridge and subsequent desalting and elution with a conventional high‐performance liquid chromatography gradient system for analysis
ArticleAbstract: Isolation of proteins from polyacrylamide electrophoresis gels by a novel combination of techniquesPalabras claves:High‐performance liquid chromatography, Protein microanalysis, Reverse staining, Sodium dodecyl sulfate‐polyacrylamide gel electrophoresisAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Frank R., Madrazo J., Mendez E.Fuentes:scopusZinc-imidazole positive: A new method for DNA detection after electrophoresis on agarose gels not interfering with DNA biological integrity
ArticleAbstract: Nucleic acids separated by gel electrophoresis are commonly detected within the gel matrix with ethiPalabras claves:Detection of nucleic acids, DNA staining, Gel electrophoresis, UV damage, Zinc stainingAutores:Casalvilla R., Eugenio Hardy, Fernandez‐Patron C., Pupo E., Sosa A.E.Fuentes:scopusRecovery of biologically active proteins detected with imidazole-sodium dodecyl sulfate-zinc (reverse stain) on sodium dodecyl sulfate gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Hernandez L., Santana-Milián H., Sosa A.E.Fuentes:scopus