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scopus(6)
A procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusDouble Staining of Coomassie Blue-Stained Polyacrylamide Gels by Imidazole-Sodium Dodecyl Sulfate-Zinc Reverse Staining: Sensitive Detection of Coomassie Blue-Undetected Proteins
ArticleAbstract: The sensitivity, simplicity, and relative rapidity of Coomassie blue staining have made this techniqPalabras claves:Autores:Castellanos L.R., Eugenio Hardy, Fernandez‐Patron C., Seoane J., Sosa A.E.Fuentes:scopusHigh yield elution of proteins from sodium dodecyl sulfate- polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in-solution biological activity analysis of on-gel renatured proteins
Conference ObjectAbstract: A simple, reliable procedure for practically quantitative (90-98%) and fast (<30 min) elution of proPalabras claves:Passive elution, Protein detection, Protein micropurification, SDS PAGE, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V., Santana-Milián H.Fuentes:scopusUnderstanding the mechanism of the zinc-ion stains of biomacromolecules in electrophoresis gels: Generalization of the reverse-staining technique
ArticleAbstract: We have recently shown that a few nanograms of protein separated by electrophoresis in sodium dodecyPalabras claves:detection, Imidazole, inflammation, Lysozyme, Negative stainingAutores:Castellanos‐Serra L., Estevez E., Eugenio Hardy, Fernandez‐Patron C., Frank R., Guerra M., Mehl E.Fuentes:scopusZinc-imidazole positive: A new method for DNA detection after electrophoresis on agarose gels not interfering with DNA biological integrity
ArticleAbstract: Nucleic acids separated by gel electrophoresis are commonly detected within the gel matrix with ethiPalabras claves:Detection of nucleic acids, DNA staining, Gel electrophoresis, UV damage, Zinc stainingAutores:Casalvilla R., Eugenio Hardy, Fernandez‐Patron C., Pupo E., Sosa A.E.Fuentes:scopusRecovery of biologically active proteins detected with imidazole-sodium dodecyl sulfate-zinc (reverse stain) on sodium dodecyl sulfate gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Hernandez L., Santana-Milián H., Sosa A.E.Fuentes:scopus