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Methods in Molecular Biology(3)
Archives of Biochemistry and Biophysics(2)
Frontiers in Plant Science(2)
Journal of Proteome Research(2)
Journal of Proteomics(2)
Changes of defense proteins in the extracellular proteome of grapevine (Vitis vinifera cv. Gamay) cell cultures in response to elicitors
ArticleAbstract: In plant cells, elicitors induce defense responses that resemble those triggered by pathogen attack,Palabras claves:2-DE, cell culture, Chitinase-III, Class III secretory basic peroxidase, Extracellular proteome, GrapevineAutores:Martínez R., Martínez-Esteso M.J., Pedreño M.A., Susana Sellés-Marchart, Vera-Urbina J.C.Fuentes:scopusA focused multiple reaction monitoring (MRM) quantitative method for bioactive grapevine stilbenes by ultra-high-performance liquid chromatography coupled to triple-quadrupole mass spectrometry (UHPLC-QqQ)
ArticleAbstract: Grapevine stilbenes are a family of polyphenols which derive from trans-resveratrol having antifungaPalabras claves:BIOCONVERSIÓN, cell culture, Epsilon-viniferin, MRM, Piceatannol, Piceid, Pterostilbene, Resveratrol, UHPLC, wineAutores:Hurtado-Gaitán E., Martínez R., Martínez-Márquez A., Samper-Herrero A., Susana Sellés-MarchartFuentes:scopusA tau class glutathione-S-transferase is involved in trans-resveratrol transport out of grapevine cells
ArticleAbstract: Vitis vinifera cell cultures respond to pathogens and elicitors by synthesizing and extracellularlyPalabras claves:cell culture, DIGE proteomics, Glutathione-S-transferase, qRT-PCR, Resveratrol, Transformation, transport, Vitis viníferaAutores:Hurtado E., Jaime Morante Carriel, Martínez R., Martínez-Esteso M.J., Martínez-Márquez A., Palazon J., Susana Sellés-Marchart, Vilella-Antón M.T.Fuentes:scopusDevelopment and validation of MRM methods to quantify protein isoforms of polyphenol oxidase in loquat fruits
ArticleAbstract: Multiple reaction monitoring (MRM) is emerging as a promising technique for the detection and quantiPalabras claves:Isoform, loquat fruits, Multiple reaction monitoring, polyphenol oxidase, transitionsAutores:Jaime Morante Carriel, Luque I., Martínez R., Martínez-Esteso M.J., Martínez-Márquez A., Pineda-Lucas J.L., Susana Sellés-MarchartFuentes:scopusDIGE analysis of proteome changes accompanying large resveratrol production by grapevine (Vitis vinifera cv. Gamay) cell cultures in response to methyl-β-cyclodextrin and methyl jasmonate elicitors
ArticleAbstract: We had previously shown that Vitis vinifera cv. Gamay cell suspension accumulates extracellularly laPalabras claves:cell culture, Cyclodextrin, DIGE, Glutathione-S transferase class tau, Grapevine, Methyl jasmonate, Proteome, Resveratrol, Stilbene synthaseAutores:Martínez R., Martínez-Esteso M.J., Pedreño M.A., Susana Sellés-Marchart, Vera-Urbina J.C.Fuentes:scopusEffect of detergents, trypsin and unsaturated fatty acids on latent loquat fruit polyphenol oxidase: Basis for the enzyme's activity regulation
ArticleAbstract: The effects of detergents, trypsin and fatty acids on structural and functional properties of a purePalabras claves:Alkyl sulfates, Alkyl sulfonates, Chlorogenic acid, Fluorescence, latency, Linolenic acid, Loquat fruit, polyphenol oxidase, regulation, TrypsinAutores:Casado-Vela J., Martínez R., Susana Sellés-MarchartFuentes:scopusDifferential plant proteome analysis by isobaric tags for relative and absolute quantitation (iTRAQ)
ArticleAbstract: Protein relative quantitation is one of the main targets in many proteomic experiments. Among the raPalabras claves:Isobaric tag, iTRAQ, Liquid chromatography, Mass spectrometry, Plant proteomics, Quantitation, Strong cation exchangeAutores:Casado-Vela J., Martínez R., Martínez-Esteso M.J., Pedreño M.A., Susana Sellés-MarchartFuentes:scopusITRAQ-based profiling of grape berry exocarp proteins during ripening using a parallel mass spectrometric method
ArticleAbstract: The 4-plex iTRAQ platform was utilized to analyze the protein profiles in four stages of grapevine bPalabras claves:Autores:Casado-Vela J., Elortza F., Martínez R., Martínez-Esteso M.J., Pedreño M.A., Susana Sellés-MarchartFuentes:scopusIsolation of a latent polyphenol oxidase from loquat fruit (Eriobotrya japonica Lindl.): Kinetic characterization and comparison with the active form
ArticleAbstract: Polyphenol oxidase (PPO) has been extracted from both soluble and particulate fractions of loquat frPalabras claves:Chlorogenic acid, Kinetic characterization, latency, Loquat fruit, Membrane proteins, polyphenol oxidase, purification, SDS, Triton X-114Autores:Casado-Vela J., Martínez R., Susana Sellés-MarchartFuentes:scopusRNA isolation from loquat and other recalcitrant woody plants with high quality and yield
ArticleAbstract: RNA isolation is difficult in plants that contain large amounts of polysaccharides and polyphenol coPalabras claves:fruits, Polyphenols, polysaccharides, RNA isolation, Woody plantsAutores:Jaime Morante Carriel, Luque I., Martínez R., Martínez-Esteso M.J., Martínez-Márquez A., Susana Sellés-MarchartFuentes:scopus