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High-yield bioactive triterpenoid production by heterologous expression in Nicotiana benthamiana using the Tsukuba system
ArticleAbstract: Oleanolic acid is a pentacyclic triterpenoid found in numerous plant species and is a precursor to sPalabras claves:CYP716A12, Nicotiana benthamiana, oleanolic acid, plant-specialized metabolites, transient protein expression, TriterpenoidsAutores:Ery Odette Fukushima, Miura K., Muranaka T., Romsuk J., Seki H., Yasumoto S.Fuentes:googlescopusIdentification of key amino acid residues toward improving the catalytic activity and substrate specificity of plant-derived cytochrome P450 monooxygenases CYP716A subfamily enzyme for triterpenoid production in Saccharomyces cerevisiae
ArticleAbstract: Triterpenoids constitute a group of specialized plant metabolites with wide structural diversity andPalabras claves:bioinformatics, CYP716A, cytochrome P450 monooxygenase, in vivo functional analysis, protein engineering, triterpene oxidaseAutores:Ery Odette Fukushima, Muranaka T., Romsuk J., Seki H., Yasumoto S.Fuentes:googlescopus