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Complexity and solutions to the isolation problem of Gram negative lipopolysaccharides' bacteria molecular species
ReviewAbstract: Gram negative bacteria frequently synthesize a great number of lipopolysaccharides (LPS) molecular sPalabras claves:chromatography, Electrophoresis, Elution, Isolation, lipopolysaccharides, Mass spectrometry, Reverse stainingAutores:Eugenio Hardy, Pupo E.Fuentes:scopusCorrespondence between radioactive and functional methods in the quality control of DNA restriction and modifying enzymes
ArticleAbstract: We evaluated the use of two radiolabeled λ DNA/Hpa II substrates to detect 5'→3, 3'→5' single and doPalabras claves:Autores:González E.M., Luis E. Trujillo, Miranda F., Pérez E.R., Pupo E.Fuentes:scopusFeasibility, via a new interface, of mass spectrometry of lipopolysaccharides separated in polyacrylamide gels
ArticleAbstract: For the first time, it was demonstrated the feasibility of analyzing by mass spectrometry (MS) Gram-Palabras claves:Autores:Eugenio Hardy, Pupo E.Fuentes:scopusElution of lipopolysaccharides from polyacrylamide gels
ArticleAbstract: A simple procedure for elution in water of bacterial lipopolysaccharides (LPS) from sodium dodecyl sPalabras claves:Elution, Gel electrophoresis, LAL, Silver staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio Hardy, Guerra M., Pupo E., Santana-Milián H.Fuentes:scopusElution of unmodified oligodeoxynucleotides from zinc-Imidazole negatively stained polyacrylamide gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Pupo E., Silva J.A., Silva R.Fuentes:scopusHigh-efficiency passive elution of bacterial lipopolysaccharides from polyacrylamide gels
ArticleAbstract: We recently described a method for recovering polyacrylamide-gel- separated bacterial lipopolysacchaPalabras claves:Gel electrophoresis, lipopolysaccharides, purification, Silver staining, Zinc stainingAutores:Alonso M., Eugenio Hardy, López C.M., Pupo E.Fuentes:scopusLinking mass spectrometry and slab-polyacrylamide gel electrophoresis by passive elution of lipopolysaccharides from reverse-stained gels: Analysis of gel-purified lipopolysaccharides from Haemophilus influenzae strain rd
ArticleAbstract: Haemophilus influenzae is an important cause of human disease, and its lipopolysaccharide (LPS) is kPalabras claves:Autores:Eugenio Hardy, Gulin S., Pupo E., Schweda E.K.H.Fuentes:scopusIsolation of smooth-type lipopolysaccharides to electrophoretic homogeneity
ArticleAbstract: The high structural heterogeneity of smooth-type lipopolysaccharides (LPS) enormously complicates thPalabras claves:biological activity, Limulus amebocyte lysate, LIPOPOLYSACCHARIDEAutores:Eugenio Hardy, Pupo E.Fuentes:scopusZinc-imidazole positive: A new method for DNA detection after electrophoresis on agarose gels not interfering with DNA biological integrity
ArticleAbstract: Nucleic acids separated by gel electrophoresis are commonly detected within the gel matrix with ethiPalabras claves:Detection of nucleic acids, DNA staining, Gel electrophoresis, UV damage, Zinc stainingAutores:Casalvilla R., Eugenio Hardy, Fernandez‐Patron C., Pupo E., Sosa A.E.Fuentes:scopus