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Complexity and solutions to the isolation problem of Gram negative lipopolysaccharides' bacteria molecular species
ReviewAbstract: Gram negative bacteria frequently synthesize a great number of lipopolysaccharides (LPS) molecular sPalabras claves:chromatography, Electrophoresis, Elution, Isolation, lipopolysaccharides, Mass spectrometry, Reverse stainingAutores:Eugenio Hardy, Pupo E.Fuentes:scopusHigh-efficiency passive elution of bacterial lipopolysaccharides from polyacrylamide gels
ArticleAbstract: We recently described a method for recovering polyacrylamide-gel- separated bacterial lipopolysacchaPalabras claves:Gel electrophoresis, lipopolysaccharides, purification, Silver staining, Zinc stainingAutores:Alonso M., Eugenio Hardy, López C.M., Pupo E.Fuentes:scopusMatrix-assisted laser desorption/ionization-time of flight-mass spectrometry of lipopolysaccharide species separated by slab-polyacrylamide gel electrophoresis: High-resolution separation and molecular weight determination of lipooligo-saccharides from Vibro fischeri strain HMK
ArticleAbstract: We recently demonstrated that the combined use of lipopolysaccharide (LPS) reverse staining and highPalabras claves:Elution, Gel electrophoresis, LIPOPOLYSACCHARIDE, Mass spectrometry, Reverse stainingAutores:Apicella M.A., Eugenio Hardy, Gibson B.W., Phillips N.J., Pupo E.Fuentes:scopusMice immunization with gel electrophoresis-micropurified bacterial lipopolysaccharides
ArticleAbstract: Some evidence on the possible use of sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDSPalabras claves:Elution, Gel electrophoresis, Immunization, lipopolysaccharides, Zinc stainingAutores:Aguila A., Castellanos‐Serra L., Eugenio Hardy, Núñez J.F., Pupo E., Santana-Milián H.Fuentes:scopusSensitive reverse staining of bacterial lipopolysaccharides on polyacrylamide gels by using zinc and imidazole salts
ArticleAbstract: We present a new method for visualizing bacterial lipopolysaccharides (LPS)/lipooligosaccharides (LOPalabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Pupo E., Reyes J.Fuentes:scopus