Mostrando 10 resultados de: 10
Filtros aplicados
Publisher
Electrophoresis(3)
Analytical Biochemistry(2)
Biotecnologia Aplicada(2)
Analytical Chemistry(1)
Journal of Controlled Release(1)
Área temáticas
Microorganismos, hongos y algas(3)
Química orgánica(2)
Biología(1)
Ecología(1)
Fisiología y materias afines(1)
Área de conocimiento
Bioquímica(7)
Biología molecular(3)
Biotecnología(2)
Biomedicina(1)
Microbiología(1)
Origen
scopus(10)
Comparison of two gel electrophoresis-based DNA micropurification methods for molecular cloning
ArticleAbstract: We recently described a method for recovering nucleic acids separated from polyacrylamide gels, whicPalabras claves:Cloning, Ethidium bromide, Gel electrophoresis, Ultraviolet-light, Zinc stainingAutores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Hermida L., Niebla O., Pupo E., Silva R.Fuentes:scopusComplexity and solutions to the isolation problem of Gram negative lipopolysaccharides' bacteria molecular species
ReviewAbstract: Gram negative bacteria frequently synthesize a great number of lipopolysaccharides (LPS) molecular sPalabras claves:chromatography, Electrophoresis, Elution, Isolation, lipopolysaccharides, Mass spectrometry, Reverse stainingAutores:Eugenio Hardy, Pupo E.Fuentes:scopusCorrespondence between radioactive and functional methods in the quality control of DNA restriction and modifying enzymes
ArticleAbstract: We evaluated the use of two radiolabeled λ DNA/Hpa II substrates to detect 5'→3, 3'→5' single and doPalabras claves:Autores:González E.M., Luis E. Trujillo, Miranda F., Pérez E.R., Pupo E.Fuentes:scopusElution of lipopolysaccharides from polyacrylamide gels
ArticleAbstract: A simple procedure for elution in water of bacterial lipopolysaccharides (LPS) from sodium dodecyl sPalabras claves:Elution, Gel electrophoresis, LAL, Silver staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio Hardy, Guerra M., Pupo E., Santana-Milián H.Fuentes:scopusElution of unmodified oligodeoxynucleotides from zinc-Imidazole negatively stained polyacrylamide gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Pupo E., Silva J.A., Silva R.Fuentes:scopusNegative staining with zinc-imidazole of gel electrophoresis-separated nucleic acids
ArticleAbstract: Zinc and imidazole salts were applied for the detection of nucleic acids on either polyacrylamide orPalabras claves:Agarose gel electrophoresis, Detection of nucleic acids, Negative staining, Zinc staining, Zinc-imidazole detectionAutores:Casalvilla R., Castellanos‐Serra L., Eugenio Hardy, López E., Luis E. Trujillo, Pupo E., Sosa A.E.Fuentes:scopusLinking mass spectrometry and slab-polyacrylamide gel electrophoresis by passive elution of lipopolysaccharides from reverse-stained gels: Analysis of gel-purified lipopolysaccharides from Haemophilus influenzae strain rd
ArticleAbstract: Haemophilus influenzae is an important cause of human disease, and its lipopolysaccharide (LPS) is kPalabras claves:Autores:Eugenio Hardy, Gulin S., Pupo E., Schweda E.K.H.Fuentes:scopusIsolation of smooth-type lipopolysaccharides to electrophoretic homogeneity
ArticleAbstract: The high structural heterogeneity of smooth-type lipopolysaccharides (LPS) enormously complicates thPalabras claves:biological activity, Limulus amebocyte lysate, LIPOPOLYSACCHARIDEAutores:Eugenio Hardy, Pupo E.Fuentes:scopusZinc-imidazole positive: A new method for DNA detection after electrophoresis on agarose gels not interfering with DNA biological integrity
ArticleAbstract: Nucleic acids separated by gel electrophoresis are commonly detected within the gel matrix with ethiPalabras claves:Detection of nucleic acids, DNA staining, Gel electrophoresis, UV damage, Zinc stainingAutores:Casalvilla R., Eugenio Hardy, Fernandez‐Patron C., Pupo E., Sosa A.E.Fuentes:scopusPreparation of plasmid DNA-containing liposomes using a high-pressure homogenization-extrusion technique
ArticleAbstract: High-pressure homogenization-extrusion (HPHE) is a method that can be used for downsizing large lipiPalabras claves:Dehydration-rehydration, DNA delivery, EXTRUSIÓN, High-pressure homogenization, Vaccine deliveryAutores:De La Rosa M., Duarte Cano C.A., Dueñas S., Eugenio Hardy, Padrón A., Pupo E., Quintana D., Santana E., Sotolongo J.Fuentes:scopus