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Negative staining with zinc-imidazole of gel electrophoresis-separated nucleic acids
ArticleAbstract: Zinc and imidazole salts were applied for the detection of nucleic acids on either polyacrylamide orPalabras claves:Agarose gel electrophoresis, Detection of nucleic acids, Negative staining, Zinc staining, Zinc-imidazole detectionAutores:Casalvilla R., Castellanos‐Serra L., Eugenio Hardy, López E., Luis E. Trujillo, Pupo E., Sosa A.E.Fuentes:scopusHigh-efficiency passive elution of bacterial lipopolysaccharides from polyacrylamide gels
ArticleAbstract: We recently described a method for recovering polyacrylamide-gel- separated bacterial lipopolysacchaPalabras claves:Gel electrophoresis, lipopolysaccharides, purification, Silver staining, Zinc stainingAutores:Alonso M., Eugenio Hardy, López C.M., Pupo E.Fuentes:scopusMatrix-assisted laser desorption/ionization-time of flight-mass spectrometry of lipopolysaccharide species separated by slab-polyacrylamide gel electrophoresis: High-resolution separation and molecular weight determination of lipooligo-saccharides from Vibro fischeri strain HMK
ArticleAbstract: We recently demonstrated that the combined use of lipopolysaccharide (LPS) reverse staining and highPalabras claves:Elution, Gel electrophoresis, LIPOPOLYSACCHARIDE, Mass spectrometry, Reverse stainingAutores:Apicella M.A., Eugenio Hardy, Gibson B.W., Phillips N.J., Pupo E.Fuentes:scopusMice immunization with gel electrophoresis-micropurified bacterial lipopolysaccharides
ArticleAbstract: Some evidence on the possible use of sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDSPalabras claves:Elution, Gel electrophoresis, Immunization, lipopolysaccharides, Zinc stainingAutores:Aguila A., Castellanos‐Serra L., Eugenio Hardy, Núñez J.F., Pupo E., Santana-Milián H.Fuentes:scopusIsolation of smooth-type lipopolysaccharides to electrophoretic homogeneity
ArticleAbstract: The high structural heterogeneity of smooth-type lipopolysaccharides (LPS) enormously complicates thPalabras claves:biological activity, Limulus amebocyte lysate, LIPOPOLYSACCHARIDEAutores:Eugenio Hardy, Pupo E.Fuentes:scopusZinc-imidazole positive: A new method for DNA detection after electrophoresis on agarose gels not interfering with DNA biological integrity
ArticleAbstract: Nucleic acids separated by gel electrophoresis are commonly detected within the gel matrix with ethiPalabras claves:Detection of nucleic acids, DNA staining, Gel electrophoresis, UV damage, Zinc stainingAutores:Casalvilla R., Eugenio Hardy, Fernandez‐Patron C., Pupo E., Sosa A.E.Fuentes:scopus