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Constitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusFunctional production and secretion of the Gluconacetobacter diazotrophicus fructose-releasing exo-levanase (LsdB) in Pichia pastoris
ArticleAbstract: The gene encoding the fructose-releasing exo-levanase (LsdB; EC 3.2.1.65) from Gluconacetobacter diaPalabras claves:Alcohol oxidase, AOX, AOX1 promoter, Gap, GAP promoter, Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus levanase, Gluconacetobacter diazotrophicus levansucrase, Glyceraldehyde 3-phosphate dehydrogenase, Levanase, LsdA, LsdB, PICHIA PASTORISAutores:Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Menéndez C.Fuentes:googlescopusHigh levan accumulation in transgenic tobacco plants expressing the Gluconacetobacter diazotrophicus levansucrase gene
ArticleAbstract: Bacterial levansucrase (EC 2.4.1.10) converts sucrose into non-linear levan consisting of long β(2,6Palabras claves:FOS, Fructan, Gluconacetobacter diazotrophicus, Levan, Levansucrase, Transgenic tobaccoAutores:Arrieta J.G., Banguela A., Hernandez L., Luis E. Trujillo, Menéndez C., Rodríguez R.Fuentes:scopusProduction of highly polymerized bacterial levan in two eukaryotic hosts of biotechnological interest
ArticleAbstract: Bacterial levan [β(2,6)-linked polyfructan] has potential applications in the food, bio-energetic, mPalabras claves:Catalytical properties, Fructan, Gluconacetobacter diazotrophicus, Levan, Levansucrase, N-glycosilation, PICHIA PASTORIS, Transgenic tobaccoAutores:Arrieta J.G., Banguela A., Borroto C., Enrique Pérez C.M., Hernandez L., Luis E. Trujillo, Pujol M., Ramírez R., Rodríguez R.Fuentes:scopus