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An experimental protocol for molecular biology lab at an Amazonian University
ArticleAbstract: Laboratory-based practical classes are an essential component in teaching molecular biology for undePalabras claves:frog secretions, laboratory exercise, Molecular cloning, PeptidesAutores:Carolina Proaño-Bolaños, Fernando Valdivieso-Rivera, José Rafael de AlmeidaFuentes:googlescopusNovel Kazal-type proteinase inhibitors from the skin secretion of the Splendid leaf frog, Cruziohyla calcarifer
ArticleAbstract: Peptidase inhibitors have an important role controlling a variety of biological processes. Here, wePalabras claves:Cruziohyla calcarifer, Frog skin secretion, Kazal-type proteinase inhibitors, Molecular cloning, Peptidomic, tandem mass spectrometryAutores:Carolina Proaño-Bolaños, Chen T., Li R., Luis A. Coloma, Shaw C., Tapia E.E., Wang L., Xi X., Zhou M.Fuentes:googlescopusNovel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer
ArticleAbstract: Antimicrobial peptides (AMPs) constitute part of a broad range of bioactive compounds present on divPalabras claves:Amphibians skin secretion, Antimicrobial peptides, Cruziohyla calcarifer, Cruzioseptins, Molecular cloning, Molecular dockingAutores:Ailin Blasco-Zuñiga, Camila Reinoso, Carolina Proaño-Bolaños, Felipe Morales, Fernanda Pilaquinga F．, Giovanna Morán-Marcillo, Lorena Maribel Meneses-Olmedo, Lorena Meneses, Miryan Rivera, Sebastian CuestaFuentes:googlescopusPeptidomic approach identifies cruzioseptins, a new family of potent antimicrobial peptides in the splendid leaf frog, Cruziohyla calcarifer
ArticleAbstract: Phyllomedusine frogs are an extraordinary source of biologically active peptides. At least 8 familiePalabras claves:Antimicrobial peptides, Cruzioseptins, Molecular cloning, Peptidomic, Skin secretions, tandem mass spectrometryAutores:Carolina Proaño-Bolaños, Chen T., Luis A. Coloma, Shaw C., Wang L., Zhou M.Fuentes:googlescopusSkin secretion transcriptome remains in chromatographic fractions suitable for molecular cloning
ArticleAbstract: Traditional sources of mRNA for molecular cloning on amphibian skin secretion studies have been thePalabras claves:Liquid chromatography, Molecular cloning, mRNA, Skin secretion, TranscriptomeAutores:Carolina Proaño-Bolaños, Chen T., Shaw C., Zhou M.Fuentes:googlescopus