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Novel biocatalysts for industrial fructooligosaccharides (FOS) production from fucosyltransferase and mutagenized invertase genes constitutively expressed in Komagataella phaffii (previously Pichia pastoris)
ArticleAbstract: Fructosyltransferases (FTFs) and invertases catalyze the production of short-chain fructo-oligosacchPalabras claves:1-fructosyltransferase, fructo-oligosaccharides (FOS), Fructosyltransferases, invertases, Komagataella phaffii, Mutants, Thermotoga maritimaAutores:Dubiel Alfonso-González, Enrique Rosendo Pérez Cruz, Gissela Estefanía Ponce Quesada, Hernandez L., José-Manuel Pais-Chanfrau, Luis Enrique Trujillo Toledo, Martínez D., Menéndez C., Ramírez R., Sobrino A.Fuentes:scopusFunctional production and secretion of the Gluconacetobacter diazotrophicus fructose-releasing exo-levanase (LsdB) in Pichia pastoris
ArticleAbstract: The gene encoding the fructose-releasing exo-levanase (LsdB; EC 3.2.1.65) from Gluconacetobacter diaPalabras claves:Alcohol oxidase, AOX, AOX1 promoter, Gap, GAP promoter, Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus levanase, Gluconacetobacter diazotrophicus levansucrase, Glyceraldehyde 3-phosphate dehydrogenase, Levanase, LsdA, LsdB, PICHIA PASTORISAutores:Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Menéndez C.Fuentes:googlescopusMultiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris
ArticleAbstract: We have found a direct relationship between protein production in Pichia pastoris and the number ofPalabras claves:Insulin, Multiple gene copies, PICHIA PASTORIS, Protein expressionAutores:Besada-Pérez V., Cabello C., Castellanos L.R., García L., Hernández L., Hidalgo A., Jorge Valdés, José-Manuel Pais-Chanfrau, Lamazares E., Mansur M., Martinez E., Plana L., Terrero Y., Valdés J., Varas L.Fuentes:googlescopusOptimization of fructose-rich syrups production from Opuntia ficus-indica inulin using immobilized inulinase on Luffa cylindrical
ArticleAbstract: Background: Luffa cylindrica has numerous domestic and industrial uses. For example, this natural fiPalabras claves:central composite design, Fructose-rich syrups, Immobilized enzymes, Immobilized inulinase, inulin, Inulinase, Luffa cylindrica, optimization, OPUNTIA FICUS-INDICA, production, Response Surface MethodologyAutores:AMAURY PEREZ MARTINEZ, Dayana T. Montalvo-Villacreses, González-Suaréz E., Jimmy Núñez-Pérez, José-Manuel Pais-Chanfrau, Marco Vinicio Lara Fiallos, Rosario C. Espín-ValladaresFuentes:scopusImmobilised Inulinase from Aspergillus niger for Fructose Syrup Production: An Optimisation Model
ArticleAbstract: Fructose is a carbohydrate with essential applications in the food industry, mainly due to its highPalabras claves:enzymatic inmobilisation, fructose, Inulinase, total productivityAutores:AMAURY PEREZ MARTINEZ, Jimmy Núñez-Pérez, José-Manuel Pais-Chanfrau, Juan Carlos DelaVega-Quintero, Marco Vinicio Lara Fiallos, Rosario C. Espín-Valladares, Valeria Olmedo-Galarza, Yomira T. Ayala ChamorroFuentes:scopus