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Complete sucrose hydrolysis by heat-killed recombinant Pichia pastoris cells entrapped in calcium alginate
ArticleAbstract: Background: An ideal immobilized biocatalyst for the industrial-scale production of invert sugar shoPalabras claves:Alginate, Immobilization, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Echemendia F.M., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Quintero Y., Ramírez R., Sobrino A.Fuentes:scopusAn efficient protocol for sugarcane (Saccharum spp. L.) transformation mediated by Agrobacterium tumefaciens
ArticleAbstract: This is the first successful report of the recovery of morphologically normal transgenic sugarcane pPalabras claves:Agrobacterium, SACCHARUM, TransformationAutores:Arencibia A.D., Carmona E.R., Chan M.T., Luis E. Trujillo, Oramas P., Tellez-Rodriguez P., Yu S.M.Fuentes:scopusA new area of application and research in bio-processes: Biotechnologies in civil construction
ReviewAbstract: Construction Biotechnology is a new scientific and engineering discipline that has been developing ePalabras claves:BIOGENIC, Biomaterials, Biotechnological, cement, concrete, MicroorganismsAutores:A. Barberán, A. Cajas, D. Chávez, José-Manuel Pais-Chanfrau, Luis E. Trujillo, M. C. Egas, M. Criollo, Pineda J.Fuentes:scopusA pH shift-based procedure to screen fructoligosaccharides fermenting yeast or bacterial strains
ArticleAbstract: Symbiotic, a combination of short chains-carbohydrates with prebiotic properties e.g. fructooligosacPalabras claves:Bromothymol blue, Fructoligosaccharides, Fructoligosaccharides fermenting microorganisms, prebiotics, probiotics, screening, SymbioticAutores:Arrieta J.G., Brizuela M.A., Hernandez L., Luis E. Trujillo, Pérez E.R., Pérez H., Trujillo G.Fuentes:scopusA protocol for the purification of bovine serum albumin free of deoxyribonuclease activity
ArticleAbstract: Bovine serum albumin, free of deoxyribonuclease activity, was obtained in our laboratory using ion-ePalabras claves:Autores:Castellanos L.R., Garcia O., Herrera L., Luis E. TrujilloFuentes:scopusA type II protein secretory pathway required for levansucrase secretion by Gluconacetobacter diazotrophicus
ArticleAbstract: The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levanPalabras claves:Autores:Alfonso D., Arrieta J.G., Hernandez L., Luis E. Trujillo, Menéndez C., Ramírez R., Soto M., Sotolongo M.Fuentes:scopusDetection of deoxyribonuclease activities using a PCR-generated radiolabeled DNA substrate
ArticleAbstract: Here we evaluated a radioactive DNA substrate for the efficient detection of deoxyribonuclease activPalabras claves:Deoxyribonuclease activity, PCR, Quality controlAutores:Brito J., Dafhnis F., Luis E. Trujillo, Miranda F., Pérez E.R.Fuentes:scopusDevelopment of soluble and immobilized Biocatalysts based on a recombinant thermostable β-fructosidase enabling complete sucrose inversion at pasteurization temperatures
ArticleAbstract: Biocatalysts for the industrial production of invert sugar are preferred to stably operate at high sPalabras claves:Immobilization, Invert syrup, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Basabe L., Cutiño-Ávila B.V., Del Monte-Martínez A., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Ramírez R., Sobrino A.Fuentes:scopusConstitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusConstitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris
ArticleAbstract: Enzymes for use in the sugar industry are preferred to be thermotolerant. In this study, a syntheticPalabras claves:Fructosidase, GAP promoter, Invert sugar, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:González E.M., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Pérez E.R.Fuentes:scopus