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High levan accumulation in transgenic tobacco plants expressing the Gluconacetobacter diazotrophicus levansucrase gene
ArticleAbstract: Bacterial levansucrase (EC 2.4.1.10) converts sucrose into non-linear levan consisting of long β(2,6Palabras claves:FOS, Fructan, Gluconacetobacter diazotrophicus, Levan, Levansucrase, Transgenic tobaccoAutores:Arrieta J.G., Banguela A., Hernandez L., Luis E. Trujillo, Menéndez C., Rodríguez R.Fuentes:scopusHigh level resistance to phytopathogenic fungi and oomycetes conferred by the use of a novel anti-microbial peptide
ArticleAbstract: Fungi and oomycete-borne plant disease constitute the largest limiting factor for crop productivityPalabras claves:Autores:Ayra C., Borras-Hidalgo O., Borroto C., Castillo J., Chacón O., Enriquez G.A., Gallo A., González E.M., López Y., Luis E. Trujillo, Oliva O., Portieles R., Pujol M., Rodrígues R., Rodriguez M., Simón M.Fuentes:scopusEngineering drought and salt tolerance in plants using SodERF3, a novel sugarcane ethylene responsive factor
ArticleAbstract: The ability of plants to tolerate salt and drought conditions is crucial for agricultural productionPalabras claves:Autores:Arrieta J.G., Banguela A., Borras-Hidalgo O., Coll Y., Hernández I., Hernandez L., Luis E. Trujillo, Menéndez C., Ochogavía M.E., Ramírez R., Rodríguez R.Fuentes:scopusFrom the laboratory to the industry: Enzymatic production and applications of shortchain fructooligosaccharides (FOS). Recent advances and current perspectives
ReviewAbstract: The production of high yields of oligosaccharides of specific chain length from simple raw materialsPalabras claves:Enzymatic production, Enzymes, oligosaccharidesAutores:L. K. Ponce, Luis E. Trujillo, Rodrigo Ávalos, Thelvia I. Ramos Gómez, Vanessa E. MarcilloFuentes:scopusFructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris
ArticleAbstract: Levansucrase (LsdA) (EC 2.4.1.10) from Gluconacetobacter diazotrophicus (formerly Acetobacter diazotPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Levansucrase, PICHIA PASTORIS, recombinant expressionAutores:Arrieta J.G., Dafhnis F., García J., Hernandez L., Luis E. Trujillo, Perez M., Támbara Y., Valdés J.Fuentes:scopusFructooligosaccharides production by Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris
ArticleAbstract: The non-saccharolytic yeast Pichia pastoris was engineered to express constitutively the mature regiPalabras claves:1-kestose, FOS, Fructooligosaccharides, Fructosyltransferase, GAP promoter, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Musacchio Lasa A., Pérez E.R., Pimentel E., Ramírez R., Sobrino A.Fuentes:scopusFructooligosaccharides production by immobilized Pichia pastoris cells expressing Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase
ArticleAbstract: Fructooligosaccharides (FOSs)—fructose-based oligosaccharides—are typical prebiotics with health-proPalabras claves:Calcium alginate, Cell immobilization, Fructooligosaccharides, Fructosyltransferase, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Pimentel E., Ramírez R., Rodríguez I., Sobrino A.Fuentes:scopusFructosyltransferases and invertases: Useful enzymes in the food and feed industries
Book PartAbstract: Enzymes are very popular and well-established biotechnology products with a great and growing globalPalabras claves:Bioprocess, Cell immobilization, Enzymes, FOS, GOS, Inverted sugarAutores:Cruz E.P., García D.M., Jimmy Núñez Pérez, José-Manuel Pais-Chanfrau, Leonor Margarita Rivera Intriago, Luis E. TrujilloFuentes:scopusConstitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusConstitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris
ArticleAbstract: Enzymes for use in the sugar industry are preferred to be thermotolerant. In this study, a syntheticPalabras claves:Fructosidase, GAP promoter, Invert sugar, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:González E.M., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Pérez E.R.Fuentes:scopus