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Compositional and structural characterization of monolayers and bilayers composed of native pulmonary surfactant from wild type mice
ArticleAbstract: This work comprises a structural and dynamical study of monolayers and bilayers composed of native pPalabras claves:fluorescence microscopy, LAURDAN GP function, Liquid immiscibility, Membrane domains, Native pulmonary surfactant membranesAutores:Berzina Z., De La Serna J.B., Ejsing C.S., Hannibal-Bach H.K., Hansen S., Knudsen J., L. A. Bagatolli, Simonsen A.C.Fuentes:scopusDirect visualization of the lateral structure of giant vesicles composed of pseudo-binary mixtures of sulfatide, asialo-GM1 and GM1 with POPC
ArticleAbstract: We compared the lateral structure of giant unilamellar vesicles (GUVs) composed of three pseudo binaPalabras claves:Asialo-GM1, Giant unilamellar vesicles, GM1, LAURDAN GP, Membrane domains, Membrane hydration, Sphingolipids, SulfatideAutores:L. A. Bagatolli, Maggio B., Rodi P.M.Fuentes:scopusFluid domain patterns in free-standing membranes captured on a solid support
ArticleAbstract: We devise a methodology to fixate and image dynamic fluid domain patterns of giant unilamellar vesicPalabras claves:AFM, Domains, Giant unilamellar vesicles, membranes, RaftsAutores:Bhatia T., Husen P., Ipsen J.H., L. A. Bagatolli, Simonsen A.C.Fuentes:scopusEnzymatic studies on planar supported membranes using a widefield fluorescence LAURDAN Generalized Polarization imaging approach
ArticleAbstract: We introduce a custom-built instrument designed to perform fast LAURDAN Generalized Polarization (GPPalabras claves:DiIC 18, Epitactic coupling, LAURDAN fluorescence, Lipid flip-flop, Membrane asymmetry, Membrane domains, Sphingomyelinase DAutores:Brewer J.R., L. A. Bagatolli, Stock R.P., Thoke H.S.Fuentes:scopusImpact of macromolecular crowding on the mesomorphic behavior of lipid self-assemblies
ArticleAbstract: Using LAURDAN fluorescence we observed that water dynamics measured at the interface of DOPC bilayerPalabras claves:LAURDAN fluorescence, Lipid membrane hydration, Lipid polymorphism, Macromolecular crowding, PEG, Protein secondary structureAutores:L. A. Bagatolli, Mangiarotti A.Fuentes:scopusImpact of membrane-anchored fluorescent probes on the mechanical properties of lipid bilayers
ArticleAbstract: Fluorescent probes are used in membrane biophysics studies to provide information about physical proPalabras claves:Bending elasticity, fluorescent probes, GUV, Lipid bilayer, Membrane mechanics, Vesicle fluctuations analysisAutores:Bouvrais H., Ipsen J.H., L. A. Bagatolli, Méléard P., Pott T.Fuentes:scopusMacroscopic domain formation during cooling in the platelet plasma membrane: An issue of low cholesterol content
ArticleAbstract: There has been ample debate on whether cell membranes can present macroscopic lipid domains as prediPalabras claves:cholesterol, FTIR, Lipid domain formation, Lipid membrane thermodynamics, Lipid platform, Lipid raft, Liquid-ordered phase, plateletAutores:Bali R., Crowe J., L. A. Bagatolli, Leidy C., Ramirez D., Savino L., Tablin F., Tsvetkova N.Fuentes:scopusMeasuring molecular order for lipid membrane phase studies: Linear relationship between Laurdan generalized polarization and deuterium NMR order parameter
ArticleAbstract: Two dimensional phase separation in lipid membranes and cell membranes is of interest to biology becPalabras claves:Deuterium nuclear magnetic resonance spectroscopy, fluorescence microscopy, Laurdan, Lipid model membrane, Liquid disordered-liquid ordered coexistence, Membrane orderAutores:Brewer J.R., L. A. Bagatolli, Leung S.S.W., Thewalt J.L.Fuentes:scopusMicroscopy imaging of membrane domains
OtherAbstract:Palabras claves:Autores:L. A. BagatolliFuentes:scopusPreparing giant unilamellar vesicles (GUVs) of complex lipid mixtures on demand: Mixing small unilamellar vesicles of compositionally heterogeneous mixtures
ArticleAbstract: Giant unilamellar vesicles (GUVs) are simple model membrane systems of cell-size, which are instrumePalabras claves:Confocal fluorescence microscopy, Domains, Giant unilamellar vesicles, Lipid heterogeneity, membranes, RaftsAutores:Bhatia T., Brewer J.R., Hansen P.L., Husen P., Ipsen J.H., L. A. Bagatolli, Mouritsen O.G.Fuentes:scopus