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Biotecnologia Aplicada(4)
Applied Microbiology and Biotechnology(1)
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Complete sucrose hydrolysis by heat-killed recombinant Pichia pastoris cells entrapped in calcium alginate
ArticleAbstract: Background: An ideal immobilized biocatalyst for the industrial-scale production of invert sugar shoPalabras claves:Alginate, Immobilization, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Echemendia F.M., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Quintero Y., Ramírez R., Sobrino A.Fuentes:scopusA type II protein secretory pathway required for levansucrase secretion by Gluconacetobacter diazotrophicus
ArticleAbstract: The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levanPalabras claves:Autores:Alfonso D., Arrieta J.G., Hernandez L., Luis E. Trujillo, Menéndez C., Ramírez R., Soto M., Sotolongo M.Fuentes:scopusDevelopment of soluble and immobilized Biocatalysts based on a recombinant thermostable β-fructosidase enabling complete sucrose inversion at pasteurization temperatures
ArticleAbstract: Biocatalysts for the industrial production of invert sugar are preferred to stably operate at high sPalabras claves:Immobilization, Invert syrup, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Basabe L., Cutiño-Ávila B.V., Del Monte-Martínez A., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Ramírez R., Sobrino A.Fuentes:scopusConstitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris
ArticleAbstract: Enzymes for use in the sugar industry are preferred to be thermotolerant. In this study, a syntheticPalabras claves:Fructosidase, GAP promoter, Invert sugar, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:González E.M., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Pérez E.R.Fuentes:scopusFructooligosaccharides production by Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris
ArticleAbstract: The non-saccharolytic yeast Pichia pastoris was engineered to express constitutively the mature regiPalabras claves:1-kestose, FOS, Fructooligosaccharides, Fructosyltransferase, GAP promoter, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Musacchio Lasa A., Pérez E.R., Pimentel E., Ramírez R., Sobrino A.Fuentes:scopusFructooligosaccharides production by immobilized Pichia pastoris cells expressing Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase
ArticleAbstract: Fructooligosaccharides (FOSs)—fructose-based oligosaccharides—are typical prebiotics with health-proPalabras claves:Calcium alginate, Cell immobilization, Fructooligosaccharides, Fructosyltransferase, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Pimentel E., Ramírez R., Rodríguez I., Sobrino A.Fuentes:scopusFunctional production and secretion of the Gluconacetobacter diazotrophicus fructose-releasing exo-levanase (LsdB) in Pichia pastoris
ArticleAbstract: The gene encoding the fructose-releasing exo-levanase (LsdB; EC 3.2.1.65) from Gluconacetobacter diaPalabras claves:Alcohol oxidase, AOX, AOX1 promoter, Gap, GAP promoter, Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus levanase, Gluconacetobacter diazotrophicus levansucrase, Glyceraldehyde 3-phosphate dehydrogenase, Levanase, LsdA, LsdB, PICHIA PASTORISAutores:Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Menéndez C.Fuentes:googlescopusEngineering drought and salt tolerance in plants using SodERF3, a novel sugarcane ethylene responsive factor
ArticleAbstract: The ability of plants to tolerate salt and drought conditions is crucial for agricultural productionPalabras claves:Autores:Arrieta J.G., Banguela A., Borras-Hidalgo O., Coll Y., Hernández I., Hernandez L., Luis E. Trujillo, Menéndez C., Ochogavía M.E., Ramírez R., Rodríguez R.Fuentes:scopusIdentification and recombinant expression of a bacterial exolevanase useful for the production of high fructose syrups
ArticleAbstract: The sugarcane endophyte Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted lPalabras claves:Autores:Alfonso D., Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Menéndez C., Ramírez R.Fuentes:googlescopusLevansucrase activity but not fructan accumulation in transgenic lsdA-expressing sugarcane recovered by optimized microprojectile bombardment of embryogenic calli
ArticleAbstract: Sugarcane (Saccharum spp. hybrid) emerges as an ideal crop for the cost-effective transgenic productPalabras claves:eGFP, Fructan, Genetic transformation, Levan, Levansucrase, LsdA, SugarcaneAutores:Arrieta J.G., Banguela A., Hernandez L., Kairúz E., Luis E. Trujillo, Menéndez C., Rodríguez R.Fuentes:scopus