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Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method
ArticleAbstract: A method for the preparation of new aminated agarose gels containing monoaminoethyl-N-aminoethyl strPalabras claves:Agarose gel, carboxyl coupling, monoaminoethyl-N-aminoethyl structures, protein immobilization, β-galactosidaseAutores:Bastida A., Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M., Rodríguez V., Santana C., Soler G.Fuentes:scopusStrategies for enzyme stabilization by intramolecular crosslinking with bifunctional reagents
ArticleAbstract: A original strategy to obtain intramolecular crosslinking is discussed. This strategy consisted of tPalabras claves:chemical modification of proteins, Formaldehyde, GLUTARALDEHYDE, Penicillin G acylase, Protein crosslinking, Stabilization of enzymesAutores:Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M., Rodríguez V.Fuentes:scopusSyntheses of pharmaceutical oligosaccharides catalyzed by immobilized-stabilized derivatives of different β-galactosidases
ArticleAbstract: We have developed strategies for immobilizing and stabilizing enzymes with very different and complePalabras claves:Biocatalysis, enzyme immobilization-stabilization, Enzymes, oligosaccharides, Organic synthesis, regioselectivity, β-galactosidasesAutores:Bastida A., Cristina M. Rosell, Fernández-Mayoralas A., Fernandez‐Lafuente R., Guisan J.M., Loṕez R., Martín-Lomas M., Rodríguez V., Santana C., Soler G.Fuentes:scopus