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High yield elution of proteins from sodium dodecyl sulfate- polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in-solution biological activity analysis of on-gel renatured proteins
Conference ObjectAbstract: A simple, reliable procedure for practically quantitative (90-98%) and fast (<30 min) elution of proPalabras claves:Passive elution, Protein detection, Protein micropurification, SDS PAGE, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V., Santana-Milián H.Fuentes:scopusRecovery of biologically active proteins detected with imidazole-sodium dodecyl sulfate-zinc (reverse stain) on sodium dodecyl sulfate gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Hernandez L., Santana-Milián H., Sosa A.E.Fuentes:scopus