High yield elution of proteins from sodium dodecyl sulfate- polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in-solution biological activity analysis of on-gel renatured proteins

Conference Object

Abstract: A simple, reliable procedure for practically quantitative (90-98%) and fast (<30 min) elution of pro

Palabras claves:

Passive elution, Protein detection, Protein micropurification, SDS PAGE, Trace enrichment

Autores:

Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V., Santana-Milián H.

Fuentes:

scopus