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"Reverse-staining" of biomolecules in electrophoresis gels: Analytical and micropreparative applications
ReviewAbstract: Negative or reverse staining using imidazole and zinc salts for protein detection in electrophoresisPalabras claves:Gel electrophoresis, lipopolysaccharides, Negative staining of gels, Nucleic acids, proteins, Proteomic purification, Proteomic sample preparation, Reverse staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusA procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusValidation of a mutant of the pore-forming toxin sticholysin-I for the construction of proteinase-activated immunotoxins
ArticleAbstract: The use of pore-forming toxins from sea anemones (actinoporins) in the construction of immunotoxinsPalabras claves:Actinoporin, immunotoxin, Pore-forming toxin, sea anemone cytolysin, sticholysinsAutores:Álvarez C., Calvo L., Campos J., Castellanos‐Serra L., Cilli E.M., Díaz I., Fando R., Lanio M.E., Pazos I., Pentón D., Pérez-Barzaga V., Pons T., Reytor M.L., Tejuca M., Vivian Morera CórdovaFuentes:scopusDetection of biomolecules in electrophoresis gels with salts of imidazole and zinc II: A decade of research
ReviewAbstract: The proven ability of gel electrophoresis to simultaneously resolve, in a single experiment, many coPalabras claves:Gel electrophoresis detection, Imidazole zinc staining, lipopolysaccharides, Nucleic acids, proteins, Reverse staining, reviewAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusElution of lipopolysaccharides from polyacrylamide gels
ArticleAbstract: A simple procedure for elution in water of bacterial lipopolysaccharides (LPS) from sodium dodecyl sPalabras claves:Elution, Gel electrophoresis, LAL, Silver staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio Hardy, Guerra M., Pupo E., Santana-Milián H.Fuentes:scopusElution of unmodified oligodeoxynucleotides from zinc-Imidazole negatively stained polyacrylamide gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Pupo E., Silva J.A., Silva R.Fuentes:scopusComparison of two gel electrophoresis-based DNA micropurification methods for molecular cloning
ArticleAbstract: We recently described a method for recovering nucleic acids separated from polyacrylamide gels, whicPalabras claves:Cloning, Ethidium bromide, Gel electrophoresis, ultraviolet light, Zinc stainingAutores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Hermida L., Niebla O., Pupo E., Silva R.Fuentes:scopusExpression and folding of an interleukin-2-proinsulin fusion protein and its conversion into insulin by a single step enzymatic removal of the C-peptide and the N-terminal fused sequence
ArticleAbstract: We report the expression in E. coli of a proinsulin fusion protein carrying a modified interleukin-2Palabras claves:Enzymatic processing, Proinsulin folding, Recombinant insulinAutores:Besada-Pérez V., Castellanos‐Serra L., Eugenio Hardy, Falcón-Cama V., Fernandez C., Gonzalez M., Herrera L., Perez G., Santiago Nelson Vispo, Santos-Savio A., Silva A., Ubieta R.Fuentes:scopusMice immunization with gel electrophoresis-micropurified bacterial lipopolysaccharides
ArticleAbstract: Some evidence on the possible use of sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDSPalabras claves:Elution, Gel electrophoresis, Immunization, lipopolysaccharides, Zinc stainingAutores:Aguila A., Castellanos‐Serra L., Eugenio Hardy, Juan F. Núñez, Núñez J.F., Pupo E., Santana-Milián H.Fuentes:scopusHigh yield elution of proteins from sodium dodecyl sulfate- polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in-solution biological activity analysis of on-gel renatured proteins
Conference ObjectAbstract: A simple, reliable procedure for practically quantitative (90-98%) and fast (<30 min) elution of proPalabras claves:Passive elution, Protein detection, Protein micropurification, SDS-PAGE, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V., Santana-Milián H.Fuentes:scopus