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Enzymatic studies on planar supported membranes using a widefield fluorescence LAURDAN Generalized Polarization imaging approach
ArticleAbstract: We introduce a custom-built instrument designed to perform fast LAURDAN Generalized Polarization (GPPalabras claves:DiIC 18, Epitactic coupling, LAURDAN fluorescence, Lipid flip-flop, Membrane asymmetry, Membrane domains, Sphingomyelinase DAutores:Brewer J.R., L. A. Bagatolli, Stock R.P., Thoke H.S.Fuentes:scopusMultiphoton excitation fluorescence microscopy in planar membrane systems
ArticleAbstract: The feasibility of applying multiphoton excitation fluorescence microscopy-related techniques in plaPalabras claves:Langmuir films, Laurdan, Planar supported membranes, Two-photon excitation microscopyAutores:Brewer J.R., De La Serna J.B., L. A. Bagatolli, Wagner K.Fuentes:scopusMeasuring molecular order for lipid membrane phase studies: Linear relationship between Laurdan generalized polarization and deuterium NMR order parameter
ArticleAbstract: Two dimensional phase separation in lipid membranes and cell membranes is of interest to biology becPalabras claves:Deuterium nuclear magnetic resonance spectroscopy, fluorescence microscopy, Laurdan, Lipid model membrane, Liquid disordered-liquid ordered coexistence, Membrane orderAutores:Brewer J.R., L. A. Bagatolli, Leung S.S.W., Thewalt J.L.Fuentes:scopusPreparing giant unilamellar vesicles (GUVs) of complex lipid mixtures on demand: Mixing small unilamellar vesicles of compositionally heterogeneous mixtures
ArticleAbstract: Giant unilamellar vesicles (GUVs) are simple model membrane systems of cell-size, which are instrumePalabras claves:Confocal fluorescence microscopy, Domains, Giant unilamellar vesicles, Lipid heterogeneity, membranes, RaftsAutores:Bhatia T., Brewer J.R., Hansen P.L., Husen P., Ipsen J.H., L. A. Bagatolli, Mouritsen O.G.Fuentes:scopusSpatial distribution and activity of Na <sup>+</sup>/K <sup>+</sup>-ATPase in lipid bilayer membranes with phase boundaries
ArticleAbstract: We have reconstituted functional Na +/K +-ATPase (NKA) into giant unilamellar vesicles (GUVs) of welPalabras claves:Domains, Giant unilamellar vesicles, Membrane biophysics, Membrane protein, membranes, Na /K -ATPase + +, Protein-lipid interactionAutores:Bhatia T., Brewer J.R., Cornelius F., Ipsen J.H., L. A. Bagatolli, Mouritsen O.G., Simonsen A.C.Fuentes:scopusThermotropic behavior and lateral distribution of very long chain sphingolipids
ArticleAbstract: Sphingolipids containing very long acyl chains are abundant in certain specialized tissues and minorPalabras claves:cholesterol, Differential scanning calorimetry, Fluorescence spectroscopy, Galactosylceramide, Glucosylceramide, SphingomyelinAutores:Björkqvist Y., Brewer J.R., L. A. Bagatolli, Slotte J., Westerlund B.Fuentes:scopus