Cryopreservation of Naranjilla (Solanum quitoense var. quitoense) Shoot tips by Vitrification

Abstract:

Shoot tips of in vitro germinated naranjilla (Solanum quitoense var. quitoense) plants were cryopreserved by vitrification. Shoot tips (1-3 mm) of five week-old plants were pre-cultured on Murashige and Skoog (MS) culture medium with increasing sucrose concentrations (0.4, 0.5, 0.6 and 0.7 M) for 7 days under a 16-h photoperiod and maintained at 23±2 C. Then, shoot tips were treated with a loading solution 2 M glycerol+ 0.4 M sucrose, at 26 C for 25 min in a 2 mL cryo-tube; and cryoprotected with 1 mL of pre-cooled PVS 2 solution at 4 C for 60 min. Finally, shoot tips were immersed into liquid nitrogen and kept there for 10 days. Post-warming culture of these shoot tips was carried out on a basal MS medium supplemented with 0.1 mgL-1 6-benzylaminopurine (BAP), 0.05 mgL-1 indole-3-butyric acid (IBA) and 0.1 mgL-1 gibberellic acid (GA3). The optimized protocol for S. quitoense shoot tips preservation …

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