Culture in soft agar of melanoma cells separated from human peripheral blood.

Abstract:

The purification of malignant cells from many human solid tumours has become a relatively uncomplicated procedure (Pretlow et al., 1973; Helms et al., 1976; Brattain et al., 1977; Pretlow & Pretlow, 1980, 1984; Hemstreet et al., 1980; Pretlow et al., 1984). The purification of malignant cells from the blood of patients with cancer is a much more difficult objective (Engell, 1955; Roberts et al., 1958). During the last quarter of a century, there has been comparatively little work directed towards this problem; but its solution should be facilitated by the availability of modem, selective techniques for the purification and culture of malignant cells. We studied patients with metastatic melanoma, since the identification of these malignant cells growing in soft agar is facilitated by the identification of melanin by light microscopy and melanosomes and premelanosomes by electron microscopy. Blood was drawn into heparinized …

Año de publicación:

1986

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Fuente:

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