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Culture of a carotenogenic strain of the chlorococcum sp. (Chlorophyta: Chlorococcales) isolated from rhizosphere Vicia faba (bean)

Abstract:

Chlorococcum sp. was isolated from rhizosphere of bean (Vicia faba) in a farm field in Latacunga, Cotopaxi, Ecuador, in order to evaluate the effect of inducing factors of carotenogenesis. The microalgae growth was monitored in batch culture with medium BBM. Exponentially, the higher cell density of 13,2 x 106 cel.ml-1 and chlorophyll a content, 3,65 μg.ml-1 was obtained. Instead under decrease cell, the maximum content of carotenoids was of 8,58 μml-1. The highest population of carotenogenic cells was induced with NaCl (0.5%) + light (115,2 μmol.m-2 s-1) and temperature (30 ± 2ºC) with 3,4 x 106 cel. ml-1. This strain of Chlorococcum sp. in batch cultures is able to accumulate carotenoids with age of the culture, and the combined effect of salinity, temperature and irradiancie.