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Cytogenetic characterization of Thalassophryne maculosa Günther, 1861 (Pisces: Batrachoididae) from Margarita Island, Venezuela

Abstract:

A cytogenetic analysis by conventional Giemsa staining, C-banding, G-banding, Silver staining, and Fluorescence in situ Hybridization (FISH) was carried out on Thalassophryne maculosa from Margarita Island, Venezuela. T. maculosa exhibits a diploid complement 2n = 46, consisting of 12 metacentrics, 6 submetacentrics, 20 subtelocentrics and 8 acrocentrics with an NF of 84. Constitutive heterochromatin was not abundant and was located only in the centromeric region in all chromosomes. A clear G-band pattern that aids in a better chromosome pairing was obtained. Localization of Nucleolus Organiser Regions (NORs) on the short arms of a medium-sized subtelocentric chromosome pair was detected with Ag-staining and was also confirmed by FISH, using probes of 18S rDNA (from fish) and 45S rDNA (plant origin). Copyright 2004 College of Arts And Sciences.