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Design and implementation of a prototype that allows to evaluate the anxiolytic activity in laboratory mice (Mus musculus) through open field testing using image processing

Abstract:

The Open Field model is a test used to determine the anxiolytic impact caused by substances or drugs in laboratory mice, weighing certain behavioral parameters: distance traveled, average speed, number of bowel movements, elevations, etc. The present work developed an electronic device system based on image processing, which allows automating the obtaining of the parameters of the test, which traditionally were obtained by simple observation. It began analyzing the techniques of image processing, then, the development of the electronic system is described, which is composed of an acrylic box where the mouse is located, a support for the IP camera that sends the images wirelessly to the computer, the recording program and the software developed in Matlab that processes the videos. The result is indicated through the graphical interface. The prototype was validated statistically with a group of animals giving a probability of success over 99% obtaining the parameters of the test correctly.