Detection of deoxyribonuclease activities using a PCR-generated radiolabeled DNA substrate

Abstract:

Here we evaluated a radioactive DNA substrate for the efficient detection of deoxyribonuclease activities in final preparations of DNA restriction and modifying, enzymes and other biological reagents as acetylated BSA. The substrate was obtained by radioactive PCR and it was found to be more sensitive in the detection of deoxyribonuclease activities (more than 10-5 units of a commercial DNAse 1 in 20 min of reaction) than the non-radioactive substrates commonly used for these purposes.

Año de publicación:

1999

Keywords:

• Quality control
• Deoxyribonuclease activity
• PCR

Fuente:

scopus