Detection of human Papilloma virus DNA in cervical samples in Maracaibo-Venezuela

Abstract:

Background: Cervical cancer is the second leading cause of cancer death among women worldwide, mainly in countries in process of development. There are multiple risk factors involved in the genesis of pre-invasive lesions and cancer, such as sexual behavior, and some sexually transmitted infections especially Human Papilloma Virus. Objective: To perform molecular diagnosis of HPV Methods: In a period from 2007 to 2009 have been treated 300 patients in Research Seminar in different health centers, each underwent a medical history, sampling for cytology and a citobrush (DNA collection device, Digene®), for diagnosis of HPV by PCR using primers for L1 region (MY09/11, GP5+/6+) Results: Of the patients attended 40.5% were positive for HPV infection, of which one 22.55% were diagnosed by MY09/11 primers vs 31.98% GP5+/6+ sharing between 14, 14% of cases Conclusion: The PCR evaluated in this …

Año de publicación:

2010

Keywords:

Fuente:

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