Detection of phytoplasmas associated to yellow leaf syndrome in Cuba.

Abstract:

Since the first report on yellow leaf syndrome (YLS) of sugarcane, the disease has been associated with different aetiological agents. Nowdays, it has a worldwide distribution. A study was conducted to determine the presence of phytoplasmas in sugarcane plants exhibiting typical YLS symptoms. Samples of sugarcane commercial varieties and clones from a germplasm collection were analysed using nested polymerase chain reaction (nPCR), radioactive nucleic acid hybridization (r-NAH) and transmission electron microscopy (TEM). Probes and primers based on derivative sequences of phytoplasmas 16S rRNA were used. The presence of phytoplasmas was demonstrated in 100% of all 82 sugarcane plants from different varieties showing YLS symptoms and in 85% of more than 300 samples of leaves with typical YLS symptoms (if the results are considered as individual foliar samples). Restriction fragment analysis after HaeIII digestion confirmed that PCR-amplified products of these samples had a phytoplasmal origin. No relationship was established between the presence of phytoplasmas and the type of leaf or leaf brix values. Phytoplasmas were detected in commercial sugarcane varieties, germplasm collection clones and hybrids from early stages of breeding programmes.

Año de publicación:

2000

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Fuente:

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