Determination of albuterol in plasma after aerosol inhalation by gas chromatography-mass spectrometry with selected-ion monitoring


Abstract:

Albuterol is a β2-adrenergic agonist commonly used as a bronchodilator for the treatment of patients with asthma. We have developed an assay to determine plasma levels as low as 50 pg/ml of albuterol by gas chromatography-mass spectrometry (GC-MS). This assay utilizes isotopically labeled albuterol ([13C]albuterol) as an internal standard. In this assay albuterol and the internal standard are recovered from 1 ml of plasma using solid-phase extraction. The samples are then derivatized to trimethylsilyl ethers using N,O-bis(trimethylsilyl)trifluoro-acetamid with 1% trimethylchlorosilane. The samples are then analyzed by GC-MS with selected-ion monitoring (SIM) for the ions m/z 369.15 and 370.15. The method has been validated for a concentration range of 50-10 000 pg/ml in plasma.

Año de publicación:

1997

Keywords:

  • Albuterol

Fuente:

scopusscopus

Tipo de documento:

Article

Estado:

Acceso restringido

Áreas de conocimiento:

  • Química analítica
  • Laboratorio médico

Áreas temáticas de Dewey:

  • Química analítica
  • Química inorgánica
  • Farmacología y terapéutica
Procesado con IAProcesado con IA

Objetivos de Desarrollo Sostenible:

  • ODS 3: Salud y bienestar
  • ODS 10: Reducción de las desigualdades
  • ODS 9: Industria, innovación e infraestructura
Procesado con IAProcesado con IA