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Determination of the freezing of hair rams (Pelibuey-Blackbelly) according to sperm viability and kinetics

Abstract:

The objective of this study was to categorize the freezing of sperm of 11 rams (Pelibuey-Blackbelly) aged 18 to 24 months based on total motility and sperm viability at thawing. The semen was diluted to 37 °C with Triladyl® and egg yolk, packed in 0.5 mL straws at a sperm concentration of 200x106. After 15 minutes after defrosting at 37 °C, sperm motility and kinetics were evaluated by means of the CASA system, and fluorescence viability with kit FluoVit® (Microptic, S.L., Barcelona, Spain). The freezing categorization of ram ejaculates was 27.2% and 72.7% for rams with high freezing (CAC) (n= 3) and rams with low freezing (CBC) (n= 8) respectively. Ejaculates from CAC showed significant differences (p<0.05) in kinetic speed parameters (VCL, VSL and VAP), which suggests that these ejaculates are more suitable for cryopreservation and would have a greater chance of fertilizing due to their faster and more progressive movements.