Development of Simple Sequence Repeat markers (SSR) in Annona deceptrix Westra H. Rainner (Annonaceae) endangered species in Ecuador

Abstract:

Annona deceptrix (Annonaceae) is a wild endemic endangered species. This species presents relevant attributes that man can use and be considered as a consumption option, but, its natural populations have a high degree of vulnerability, due to the loss their ecosystems caused by man. This study aimed to isolate and characterize SSR loci to determine the genetic diversity in A. deceptrix and other Annonaceae. We identi ed simple sequence repeat (SSR) regions in a microsatellite library from A. deceptrix. 22 polymorphic Simple Sequence Repeat (SSR) loci were isolated using new-generation kit Nextera of miseq illumine technology. The mean number of alleles per locus ranged from 2 to 8. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.93, respectively. These are the rst microsatellite markers developed for A. deceptrix. All SSR markers developed are promising candidates for analyzing genetic diversity within or between natural populations, also of analyzing pool genic in other species of Annona genus.

Año de publicación:

2022

Keywords:

Fuente:

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