Diagnostic techniques and sample handling
Abstract:
This chapter deals with an overview of handling of samples from "mega"-invertebrates such as tarantulas, octopus, nautilus, or smaller specimens that can be found in aquarium and terrarium collections. For aquatic animals, the client should provide a recent sample of the water and submit it in a clean container such as a plastic or glass jar with a tight seal. For terrestrial species, place the animal in a small induction chamber, or, if small enough, a well-sealed jar. Insect disease diagnostics are more developed, and clinicians can try to contact appropriate research or diagnostic facilities for assistance. One of the most difficult questions when approaching an invertebrate necropsy is determining whether the animal is actually dead. Proper fixation techniques of live specimens rely on adequate sedation of the animal. A solution of formic acid and sodium citrate can be prepared as well as well as slower decalcification solutions using Ethylenediamine Tetraacetate.
Año de publicación:
2021
Keywords:
- Invertebrate necropsy
- Insect disease diagnostics
- Ethylenediamine tetraacetate
- Fixation techniques
- Sodium citrate
- Aquatic animals
Fuente:
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Tipo de documento:
Book Part
Estado:
Acceso restringido
Áreas de conocimiento:
- Laboratorio médico
Áreas temáticas:
- Medicina y salud
- Biología
- Ingeniería química