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Diagnóstico de mycobacterium bovis en bovinos faenados en el camal de Daule durante el mes de noviembre del año 2018

Abstract:

The present investigation had as purpose to make the diagnosis of Mycobacterium bovis (M. bovis) in cattle slaughtered in the slaughterhouse in Daule. M. bovis causes bovine tuberculosis in the animal and the tuberculosis zoonotic in humans, it belongs to the group of micobacterias whose illnesses are of chronic course, the alterations that they produce not always are detected to the examination in the centers of faenamiento when the animals come in estadíos early from the illness for what it constitutes a transmission risk zoonótica that affects the public health. Its importance is underestimated and the timely diagnosis constitutes the basis for the system of epidemiological surveillance of the disease. The study consisted of taking ganglions samples retrofaríngeos and mesentéricos in bovine slaughtered in the slaughterhouse in study, that presented alterations in size and/or color in the post – mortem inspection, to be cultivated in the means of cultivation Lowenstein Jensen, Ogawa Kudoh and Stonebrink, it was realized baciloscopía using coloring of Ziehl – Neelsen to determine the presence of acidic bacillus - resistant alcohol (BAAR) and, by means of molecular skills (PCR and PCR – LAMP) to diagnose Mycobacterium spp. and M. bovis respectively. The result obtained was 43.5% positive to BAAR isolated in the three culture media, 9.7% positive to Mycobacterium spp. isolated from the culture medium Stonebrink and no positive to Mycobacterium bovis. No statistical association was found between the presence of Mycobacterium spp. and the variables sex, category zootechnical and purpose. It is recommended to carry out molecular diagnostic studies in direct samples to validate the PCR-LAMP technique as a tool for the epidemiological surveillance systems of the disease.