Diamondback moth resistance to Bacillus thuringiensis transgenic canola: Evaluation of refugia size with non-recessive resistant insects


Abstract:

Current recommendations to delay the evolution of resistance to Bacillus thuringiensis crops are that a minimum of 5-50% of a crop-growing region should include non-B. thuringiensis varieties as refuges. These recommendations are based in part on the assumption that resistance will be inherited as a recessive trait. Laboratory microcosm experiments are described with transgenic canola expressing Cry1Ac and a non-recessive Cry1Ac resistant population of the diamondback moth Plutella xylostella, in which the effect of different sizes of refugia (0%, 20% and 50%) on resistance was compared over five generations. The LC50 values for Cry1Ac increased markedly in the P. xylostella sub-populations with 0% (>100-fold) and 20% refugia (>35-fold) but showed little change (less than fivefold increase) with 50% refugia. The results support the idea that relatively high levels of refugia (non-B. thuringiensis varieties) may be required where resistance is not functionally recessive at the level of toxin expressed in the B. thuringiensis crop. © 2006 The Authors.

Año de publicación:

2006

Keywords:

  • Bacillus thuringiensis
  • Resistance management
  • refugia
  • Transgenic crop
  • Plutella xylostella

Fuente:

scopusscopus

Tipo de documento:

Article

Estado:

Acceso restringido

Áreas de conocimiento:

  • Ciencias Agrícolas

Áreas temáticas:

  • Lesiones, enfermedades y plagas de las plantas
  • Técnicas, equipos y materiales
  • Microorganismos, hongos y algas