ESTABLISHMENT OF A PAPAYA (Carica papaya L.) MICROPROPAGATION PROTOCOL IN A TEMPORARY TWIN VESSEL IMMERSION SYSTEM

Abstract:

Papaya production is affected by sexual variability in the seed. Conventional methods of propagation involve the maintenance of large land scale crops to supply the consumer's demand. Micropropagation techniques using temporary immersion of twin vessels (TIS) bioreactors are an alternative for papaya production. The objective of the study is to establish a disinfection protocol for the introduction of axillary buds and to evaluate different immersion times and inoculum density in the TIS to obtain a higher multiplication rate. Disinfection of the plant material was performed with 70% ethanol for 1 minute, 2% sodium hypochlorite for 5, 6 and 8 min, and sterile water washes. Percentage of contamination and oxidation was evaluated for 21 days. The introduction stage, Murashige & Skoog (MS) medium was used with 1 mg. L-1 of gibberellic acid and 2 mg. L-1 of kinetin. Similarly for the multiplication stage, MS medium …

Año de publicación:

2020

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