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EVALUATION OF PLA ACTIVE BIODEGRADABLE FILMS INCORPORATED OF ESSENTIAL OILS TO INHIBIT MICROBIAL ADHESION

Abstract:

An evaluation of incorporated polylactic acid (PLA) active biodegradable films of essential oils to inhibit microbial adhesion was performed using a 2V5−1 fractional exploratory design, combining the factors, amount of PEG 400 plasticizer (10 and 20 %), amount of cinnamon essential oil (0.5 % and 1 %), amount of oregano essential oil (0.5 % and 1 %), Salmonella spp. concentration (103 CFU mL−1 and 104 CFU mL−1) and Staphylococcus aureus concentration (103 CFU mL−1 and 104 CFU mL−1). The results of microbial adhesion inhibition test showed that the maximum inhibition percentage reached 73.82 ± 0.35 % corresponding to experiment 7 (bce), which contains 10 % of PEG 400, 1 % of cinnamon essential oil, 1 % of oregano essential oil, 103 CFU mL−1 of Salmonella spp. concentration, 104 CFU mL−1 of Staphylococcus aureus concentration. Statistical analysis determined that there is strong significant evidence (p − value = 0.0283) that Staphylococcus aureus concentration influences the inhibition percentage to microbial adhesion; as well as that the cinnamon essential oil- Salmonella spp. interaction has little significant evidence (p − value = 0.0711) that influences the inhibition percentage. Inhibition results greater than 60 % have the highest concentration of Staphylococcus aureus as a common factor. PLA active biodegradable films with a higher inhibition percentage can potentially be used in the food industry as a barrier mechanism to avoid bacterial contamination.