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Effect of carbon-nitrogen in the native Beauveria bassiana production by solid fermentation in Ecuador

Abstract:

The purpose of the work was to produce native Beauveria bassiana conidia at the laboratory level through Solid State Fermentation (SSF), polypropylene sleeves were used as bioreactors. The pre-cooked and sterilized substrates were: "recycled" rice and wheat, supplemented with various carbon-nitrogen sources (C-N), ratio 10: 1. The experimental design was a Categorical Multi-Factor, with 20 treatments and three repetitions to determine the variable concentration of conidia/g of bioproduct; the count of conidia was in Neubauer chamber. The mean values of controls were 1.15 x 109 Conidia/g in rice and wheat 1.22 x 109 Conidia/g with C-N supplements, the highest means of B. bassiana in rice was 1.89 x 109 Conidia/g (T7); in this same order in wheat 1.90 x 109 Conidia/g (T17). The lowest means presented: T10 and T20 (between 1.05-1.06 x 109 Conidia / g), in rice and wheat with molasses-yeast, concentration 2 (5.0-0.50 g), possibly due to an antagonistic effect between Saccharomyces cerevisiae-entomopathogenic fungi (EPF) during its cultivation. The ANOVA registered significant statistical differences (*) at ≤ p 0.05 in the factors C-N, and concentration. The Multiple Range Contrast LSD-Fisher, determined that there were no (*) between any pair of means at 95.0% confidence. It is concluded: the supplementation of C-N on rice and wheat for the mass production of B. Bassiana, influenced the yield of conidia/g of the bioproduct.