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Effect of glutamine and branched-chain amino acid supplementation on hematological and biochemical parameters of canine puppies

Abstract:

The objective of the study was to determine the influence of glutamine (GLN) and BCCAs (leucine, isoleucine and valine) on the hematological and biochemical parameters of the puppies. 31 mixed puppies of 30 days were recruited, from four litters, randomly in each litter a control group was established (without supplement) and 3 treatments: T1 (GLN 0.5 g/kg/day/PO), T2 (BCAAs, 0.25 /kg/ day/PO) and T3 (GLN 0.5 g/kg + BCAAs 0.25 g/kg/day/PO), were maintained for 90 days in a controlled environment under equal conditions, fed twice daily individually, samples were taken at 30 and 120 days of age. A randomized block design was applied using the litter as a block factor, descriptive statistics and normality tests were performed, as well as ANOVA to verify variations between treatments and tukey to position the treatments. At 30 days, the means and ranges of the hematological and biochemical parameters were similar. At 120 days in the hematological parameters, differences were determined (p<0.05) depending on the block, not the treatment (p>0.05), however, the average of leukocytes (10.33 10 * 9 /L) and the average of Neutrophils (7.17 10 * 9/L) of T2 were higher than the average of the control group and of the other treatments, showing a significant difference (p<0.05) only at a CI of 90%. The blood biochemistry parameters did not show differences (p>0.05). In conclusion, a positive effect on the innate immunity was evidenced, with BCAAs supplementation being favorable, with no evidence of toxicity.