Effect of high dose of rays-X on the parasitic action of Plasmodium berghei

Abstract:

Malaria is responsible for millions of clinical cases and approximately for 655,000 deaths annually. Therefore, the development of strategies for the design of an effective vaccine has become a priority for the Parasitologists worldwide. The aim of the present study was the evaluate the effect of irradiation with X-rays on the merozoite stage of Plasmodium berghei using the BALB/c mice as experimental model. We used doses of ionizing radiation between 80 and 350 Gy in parasitized red blood cells (GRP) from Plasmodium Berghei to determine its effect on merozoite parasites, because in previous research we showed that 50 Gy was still not sufficient to attenuate the parasite. The parasitemia was monitored daily by Giemsa stain and the effect of high dose was evaluated by indirect immunofluorescence (IFI) immunological test. Our results show that, with 120 Gy dose, 50% of individuals manage to control the infection entirely and survive. For higher dose it is observed the death of individuals even without producing significant levels of parasitemia in blood (less than 5%). IFI assay showed that for the treatments with 250, 300 and 350 Gy parasites are outside red blood cells even in the 10 day post infection. X-ray radiation dose exceeding the 250 Gy on GRP with P. berghei, diminish the ability of the parasite to invade the red blood of the host. However, the radiation has an adverse effect that causes the death of individuals for reasons unrelated to infection by malaria. Doses of radiation around 120 Gy achieve attenuation parasitic to achieving a percentage of survival of 50% in BALB/c mice.

Año de publicación:

2013

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Fuente:

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