Effect of storage temperatures of bull's epididymis on frozen-thawed sperm quality.

Abstract:

Epididymis sperm conservation is a technique widely used in animals of high genetic value as well as in wild animals dead unexpectedly. One factor that most influence in motility and sperm membrane integrity (plasma and acrosomal) as well as the fertilizing ability of epididymal spermatozoa recovered is post-mortem temperature storage of epididymis. The objective of this study was determinate sperm quality pre-freezing and post-thawed of bull epididymal sperm (Bos taurus) from the Ecuadorian Amazon recovered from testicles stored to 5 or 20 C for short-time. For this propose, ten pairs of testicles were collected from slaughterhouse. Left and right testicles of each bull were storage at 5 C (T-5 group) or 20 C (T-20 group), respectively during 6 hours (h). Sperm recovery was performed by retrograde flow of the epididymis tail and the liquid obtained was diluted in AndroMed® extender at concentration of 200 x 106 spermatozoa/mililiters mL and packed in 0.25 mL straws. Both straws T-5 (n= 30) and T-20 (n= 30) groups were frozen in vapors of nitrogen liquid and thawed at 37 C for 60 seconds (sec). The effect of storage temperature of epididymis post-mortem were assessment by ANOVA-one way. The results showed that in pre-freezing analysis, the percentage of motility (analyzed by optical microscopy), sperm vitality (V, plasma membrane integrity analyzed by eosin-nigrosine stain) and total morphological abnormalities (TA) of spermatozoa of T-5 group did no showed significant differences (P> 0.05) compared with T-20 group. In post-thawed analysis, likewise the motilities variables (assessed by CASA system, SpermVision®, Minitube …

Año de publicación:

2018

Keywords:

Fuente:

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