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Efficient preservation of DNA extracted from blood in FTA cards by Chelex method

Abstract:

Chelex resin has been developed and widely used for extracting DNA from different forensic-type samples [1]. However, no studies have shown the conservation of the extracted samples over time in samples from buccal swabs and blood in FTA cards. For this study, we tested three DNA extraction methods: Chelex 10%-Proteinase K (CHK), Phenol Chloroform (PC) and Chelex 10%-Proteinase K purified with Phenol Chloroform (CHK + PC) in two different kind of samples: blood stored over FTA cards and buccal swab samples. Beta-Actin gene (136 bp) was amplified by PCR at 0, 4 and 8 months, STR profiles and amplification of mitochondrial DNA (1200 bp) were done at 15 months to check the DNA quality. These results suggest the importance of choosing the DNA extraction method that guarantees the conservation of reference or forensic DNA samples for further studies or forensic tests.