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Alkaline hydrolysis/methylation‐acetylation: a new technique for ultrastructural DNA cytochemistry

Abstract:

A new technique for the visualization of DNA‐containing structures in electron microscopy is described. Samples of glutaraldehyde‐fixed bone marrow from rats were subjected to alkaline hydrolysis to remove RNA and the phosphate of phospho‐proteins, followed by a combined blockage of protein carboxyl and amino groups through methylation‐acetylation. After uranyl acetate staining of epoxy‐embedded ultrathin sections, chromatin from all cell types showed a highly selective and intense electron opacity. Staining methods for DNA were also positive in semithin sections. This simple procedure could be very useful in ultrastructural cytochemistry of DNA and chromatin.

Año de publicación:

1991

Keywords:

    Fuente:

    googlegoogle

    Tipo de documento:

    Other

    Estado:

    Acceso abierto

    Áreas de conocimiento:

    • Biotecnología
    • Biología molecular

    Áreas temáticas:

    • Bioquímica
    • Microorganismos, hongos y algas

    Contribuidores:

    Patricio CastilloPatricio Castillo