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Element distribution in mycelium of pisolithus arrhizus treated with cadmium dust

Abstract:

Mycelium of Pisolithus arrhizus cultured on agar media containing cadmium dust (collected from industrial electrofilters) and on media without the dust was analysed by electron spectroscopic imaging (ESI) and electron energy loss spectroscopy (EELS) connected with the transmission electron microscope. Complementary investigations of polysaccharide (PATAg) and cysteine rich protein localization were carried out. The analyses revealed abundant deposition of glycogen in the form of net-like material in the cytoplasm of the dust-supplemented mycelium, accompanied by increased levels of Ca. Heavy metals like Cd, Cu and Fe accumulated mainly in electron opaque granules and in the outer pigmented layer of the cell wall, both characterized by the presence of polysaccharides and cysteine-rich proteins. © 1994 Annals of the Botany Company.