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Amplification of the Rrn5 molecular marker in DNA extracted from callus of Stylosanthes guianensis cv. CIAT-184.

Abstract:

Several genomic DNA isolation protocols as well as the Puregene kit (Gentra System) were evaluated in lyophilized samples of leaves and callus from Stylosanthes guianensis cv. CIAT-184, in order to obtain optimum samples to amplify, through PCR technique, the Rrn5 ribosomic spacer as a possible molecular marker to detect genetic variability induced by in vitro culture. The Zhu et al. protocol [Nucleic Acids Research (1993) 21, 5279] gave an average of 500 ng/µL with the purity required for amplifying the molecular marker from 10 ng of DNA. Gel electrophoresis showed a fragment of 250 bp in all samples.

Año de publicación:

2000

Keywords:

    Fuente:

    googlegoogle

    Tipo de documento:

    Other

    Estado:

    Acceso abierto

    Áreas de conocimiento:

    • Biología molecular
    • Ciencias Agrícolas

    Áreas temáticas:

    • Microorganismos, hongos y algas
    • Bioquímica
    • Ingeniería química

    Contribuidores:

    Daynet SosaDaynet Sosa