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Estimation of common bean (Phaseolus vulgaris) leaf area by a non-destructive method

Abstract:

The aim of this study was to develop mathematical models to estimate the leaf area of common bean (Phaseolus vulgaris) in irrigated and non-irrigated water regimes from linear dimensions. An experiment was carried out in a completely randomized design with a 3×2 factorial arrangement (three cultivars: Triunfo, Garapiá and FC 104; two water regimes: irrigated and non-irrigated) with 25 replicates each. A total of 523 trifoliates were collected throughout the crop cycle. The length (L, cm) and width (W, cm) of the central leaflet of the trifoliate were measured and their product (LW) (cm2) calculated. Then, the leaf area of these trifoliates was determined by digital photography methods using ImageJ® software, and using leaf discs. The number of samples required to estimate the leaf area of a trifoliate was determined to define which method is the most accurate to be used as the real leaf area in generating equations to estimate the leaf area in common bean. The relationship between area by digital photographs and the dimensions of the central leaflet of the trifoliate (L, W and LW) was fitted by linear, quadratic and power models. Subsequently, the pbkp_redictive capacity of the equations was assessed by the root mean square error (cm2trifoliate-1), mean absolute error (cm2trifoliate-1), index of agreement and Pearson's correlation coefficient. Sample size varied between cultivars, water regimes and evaluation methods. It is more appropriate to use the leaf area provided by ImageJ® as real for comparison purposes in generating models to estimate leaf area from linear measurements, in common bean. The general equation LA = 1.092L1.945can be used in the tested regimes without accuracy losses.