An efficient protocol for isolating melanised chaetothyrialean anamorphic fungi associated with plant-ants

Abstract:

Because of their ecological characteristics, slow growth rates and the presence of contaminants, Chaetothyriales fungi associated with structures built by tropical plant-ants can be difficult to isolate with standard procedures. Here, we describe an easy-to-use protocol for obtaining pure cultures by using cotton as a first substrate. We have further found by means of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the hyphae. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Año de publicación:

2013

Keywords:

• Black fungi
• Isolation method
• Chaetothyriales
• Fluorescent staining
• Allomerus ants

Fuente:

scopus