An evaluation of siRNA screening in identification of targets in PTEN deficient tumor cells.

Abstract:

e13569 Background: PTEN is frequently lost in cancer cells through genetic mutation or epigenetic silencing. Loss of PTEN function has been widely reported to result in up-regulation of PI3K and consequently AKT signalling, therefore promoting cell survival and proliferation. More recently a role for PTEN in maintaining genomic integrity was suggested by the finding that PTEN deficient cells demonstrate chromosomal instability and are sensitive to ionising radiation induced DNA damage. Methods: We utilised a siRNA screening approach with a human ‘druggable’ genome siRNA library to identify novel drug targets for PTEN associated cancers. The library contained 2 individual siRNAs directed against 6,961 druggable targets of the human genome, including 691 kinases, 206 phosphatases, 230 DNA repair genes. Bioinformatics analysis identified 981 genes as being synthetically lethal with loss of …

Año de publicación:

2011

Keywords:

Fuente:

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