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Estrogenic activity of ethanolic extracts from leaves of ilex guayusa loes. and medicago sativa in rattus norvegicus

Abstract:

Ilex guayusa Loes. (guayusa) and Medicago sativa (alfalfa) are plants used in Ecuadorian traditional medicine to treat female infertility. It has been verified the estrogenic activity of ethanolic extracts from leaves of guayusa and alfalfa in rats (Rattus norvegicus), in the Phytochemistry Laboratory of the Sciences Faculty of the Escuela Superior Politécnica de Chimborazo (ESPOCH). The experimental methodology was the oral administration of treatments, statistically evaluated by ANOVA test (p <0.05) for multiple comparisons in a confidence interval of 99% using Tukey HSD. 25 female albino rats were divided them into five groups called: control, standard and experimental subjects D1, D2 and D3. During treatment received: saline solution (negative control), standardized commercial soy isoflavone extract (positive control) 3,64mg/kg weight, and experimental subjects three doses of 9 mg / kg, 18 mg / kg and 36 mg / kg of guayusa and alfalfa extracts respectively. After treatment serum oestradiol levels were significantly increased for the tested doses, like the ovaries and uteri weights. It is concluded that the three treatments of guayusa and alfalfa extracts produce estrogenic effect in rats both oestradiol levels and weights of reproductive organs.