1104 Transcriptome analysis reveals fundamental differences between liver of neonatal calves and transition dairy cows

Abstract:

Primary hepatocytes isolated from neonatal calf liver have been used to infer aspects of liver metabolism of dairy cows, with the assumption that physiological responses of neonatal hepatocytes mimic those of cows. To evaluate more directly the usefulness of calf hepatocytes as model to study cow responses, in particular during the early postpartal period, liver RNA from 7 Holstein cows (20 d in milk) and 7 Holstein calves (4-d old) was used for transcriptome analysis. Individual samples were used to determine expression of 7 key metabolic genes via quantitative RT-PCR. Data were log2 normalized and subjected to ANOVA using the Proc MIXED procedure of SAS. Compared with calves, the expression of genes related with lipoprotein synthesis (APOB, P = 0.01; MTTP, P = 0.01), gluconeogenesis (FBP1, P = 0.07), fatty acid oxidation (CPT1A, P = 0.01), and methionine metabolism (MAT1A, P = 0.03; BHMT, P …

Año de publicación:

2016

Keywords:

Fuente:

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