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15-Epi-lipoxin A<inf>4</inf> inhibits the progression of endometriosis in a murine model

Abstract:

Objective: To examine the pro-resolution actions of 15-epi-lipoxin A4 (LXA4) on endometriotic lesions, on the concentrations and activities of matrix metalloproteinases (MMP-2 and MMP-9) in murine endometriosis. Design: Prospective, vehicle-controlled experimental study. Setting: Animal research facility. Animal(s): BALB/c mice. Intervention(s): Endometriosis (EM) was induced in 30 mice. Fifteen of them were administered LXA4 for 24 days (LXA4 group), whereas the other 15 served as a control group (EM group). Another 15 sham-operated mice (sham-operated group) were treated with vehicles. Main Outcome Measure(s): The weight of the endometriotic lesions was measured. The concentrations, mRNA, and activities of MMP-2 and MMP-9 were determined by enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction, and gelatin zymography, respectively. Result(s): Compared with EM group, the weight of the endometriotic lesions was decreased, the concentrations of MMP-2 and MMP-9 dropped, the mRNA levels of MMP-2 and MMP-9 in the peritoneal fluid cells and the endometriotic lesions were reduced, and the activities of MMP-2 and MMP-9 were inhibited in the LXA4 group. Conclusion(s): LXA4 may inhibit the progression of endometriosis possibly by lowering the concentrations and the activities of MMP-2 and MMP-9. © 2010 American Society for Reproductive Medicine.