1603 Immunometabolic gene expression in blood neutrophils (PMN) in Holstein dairy cows supplemented with rumen-protected methionine or rumen-protected choline during the …

Abstract:

Objectives of this study were to evaluate mRNA expression of 30 genes related to neutrophil adhesion, chemotaxis and migration, oxidative stress, Toll-like receptor pathway, methionine cycle, and glutathione metabolism in response to rumen-protected methionine (MET) and choline (CHO) supplementation during the peripartal period. Forty multiparous Holstein cows were used in a randomized complete block design with 2 × 2 factorial arrangement of MET and CHO level (with or without). Treatments were control (CON), no MET or CHO; CON + MET (SMA); CON + CHO (REA); and CON + MET + CHO (MIX). From −21 d (close-up) to 30 d after calving, cows received the same diet (1.52 Mcal NEL/kg DM) from close-up to calving. From calving to 30 d, cows were on the same diet (1.71 Mcal NEL/kg DM) and continued to receive the same treatments through 30 d. MET supplementation was adjusted daily at a …

Año de publicación:

2016

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Fuente:

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